FAQ - Frequently asked questions

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Can all adherent cell lines be cultivated in the CellScrew®?

The CellScrew® is TC-treated and has been tested with various cell lines, such as mouse fibroblasts (L-929), human kidney cells (HEK-293), and stem cells (human mesenchymal stromal cells). The adhesion rates, growth rates, and final cell densities observed were comparable to or better than those achieved on standard polystyrene surfaces. For more information on testing HEK-293 cells on the PLA surface and the CellScrew®, please refer to this Application Note.
Can cells be visualized under the microscope in the CellScrew®?

The CellScrew® cannot be microscoped, similar to most other expansion systems that exceed a growth surface of ~300 cm².
Do I need to buy additional equipment to use the CellScrew®?

The CellScrew® is handled just as a regular roller bottle, where it is placed in an incubator on a roller device. However, it is crucial to position the roller bottle at a 10-15 degree angle on the roller to ensure the filter cap remains dry and the system operates effectively. For simultaneous cultivation of three CellScrew® mini in the dimensions of a CellScrew® 6K, the first order of our bundle includes the CellScrew®mini TriDock. Find more instructions on the set-up of the CellScrew® on our Handling page and in our CellScrew® Expert Series.
Is the CellScrew® sterile?

Yes, the CellScrew® comes fully sterilized, as it is gamma irradiated. We strongly advise against autoclaving the CellScrew® as it can cause damage to the product's structure and integrity.
Can the CellScrew® be used with coating?

Yes, the CellScrew® works with all standard coatings for adherent cells, including those used for cultivation of stem cells such as iPSCs or MSCs.