Large cell culture surface.
Small footprint.
Tired of dealing with many single vessels for your adherent cell culture? Combine your surfaces in one vessel and streamline your cultivation on a small footprint.
Same growth surface area
CellScrew® mini with 850 cm² compared to a T75-flask
(75 cm²)
Less vessels -
less lab work
The CellScrew® mini offers a growth surface twelve times larger than that of a T-75 flask while maintaining comparable external dimensions.
Operating one vessel instead of twelve not only reduces manual handling steps, required liquids and lab space, but also decreases the risk of contamination.
The genius in the bottle
The innovative internal structure of the CellScrew® forms a large growth surface on a small footprint and fosters optimal growth conditions with low shear forces and excellent gas exchange.
Constant and homogeneous supply with oxygen and nutrients facilitate cell growth while limiting unwanted cell differentiation.
Archimedes' screws:
excellent mixing and consistent medium supply under low shear forces
Central tube: constant flow of the liquids and liquid handling using a serological pipette
Concentric cyclinders:
form a large surface area on a small footprint
Harvest your cells in one batch and reduce trypsin by up to
66 %
Reduce cultivation reagents
Tests with HEK-293 cells showed harvest efficiencies in the CellScrew® of over 90% - with 66% less trypsin.
Excellent growth conditions
The CellScrew® mini features the same advantages as our large-scale models: The internal structure creates a large growth surface area, provides excellent oxygenation, and facilitates low-shear mixing on a small footprint.
Here is how it works
Learn the full procedure from inoculation of the CellScrew® (10K) to harvesting your cells. The described work procedure is applicable for all our CellScrew® models.
Model | CellScrew® 6K | CellScrew® 10K | CellScrew® mini |
|---|---|---|---|
Growth area | 6,000 cm² | 10,000 cm² | 850 cm² |
Media volume | 250 - 800 mL | 450 - 1,300 mL | 50 - 120 mL |
Recommended media volume | 600 mL | 1,000 mL | 85 mL |
Height | 19.19 cm | 28.39 cm | 16.78 cm |
Diameter | 12.1 cm | 12.1 cm | 5.3 cm |
Weight | 550 g | 860 g | 120 g |
Recommended speed | 0.2 to 2 rpm | 0.2 to 2 rpm | 0.2 to 2 rpm |
Mixing times (@ 0.5 rpm) | < 9 min | < 27 min | < 8 min |
kLA (@0.5 rpm) | 1.95 h-1 | 1.95 h-1 | N/A |
Sterilization | sterile (gamma irradiated), not autoclavable | sterile (gamma irradiated), not autoclavable | sterile (gamma irradiated), not autoclavable |
Material | PLA (polylactic acid), TC-treated | PLA (polylactic acid), TC-treated | PLA (polylactic acid), TC-treated |
Grow with your needs
The CellScrew® mini (850 cm²) is our small scale model for early stages of adherent cell expansion and process optimization. The CellScrew® is also available with a growth surface of 6,000 (6K) or 10,000 cm² (10K) for large-scale cultivations.
Resource-conscious cell cultivation
Laboratories are among the most resource-intensive facilities, consuming up to 10 times more energy than office buildings and accounting for up to 60% of carbon emissions in research institutions.⁴
The CellScrew® not only conserves your resources but also those of our planet. Through material-efficient additive manufacturing and the use of plant-based materials, the CellScrew® boasts a 90% lower carbon footprint than other adherent cell cultivation systems.
CellScrew®
Common systems
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Can all adherent cell lines be cultivated in the CellScrew®?The CellScrew® has been tested with various cell lines, such as mouse fibroblasts (L-929), human kidney cells (HEK-293), and stem cells (human mesenchymal stromal cells). The adhesion rates, growth rates, and final cell densities observed were comparable to or better than those achieved on standard polystyrene surfaces. For more information on the testing of HEK-293 cells on the PLA surface and the CellScrew®, please refer to this Application Note.
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Does the CellScrew® have a smaller carbon footprint compared to standard cell culture devices?Yes! The CellScrew® is made from polylactic acid (PLA), which is derived from the polymerization of lactic acid produced from plants. Our additive manufacturing process allows us to produce the same growth surface area with up to 80% less material compared to injection molded culture systems. To comprehensively assess the carbon footprint of the CellScrew®, we are currently conducting a life cycle analysis.
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Is the CellScrew® GMP-certified?The CellScrew® is currently research grade, but we aim to achieve GMP compliance by the end of Q1 2024 and are in the process of constructing a GMP-compliant facility to support this goal.
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Can cells be visualized under the microscope in the CellScrew®?The CellScrew® cannot be microscoped, similar to most other expansion systems that exceed a growth surface of ~300 cm². However, we are currently developing a fully automated, closed production platform for the cellScrew. This platform will allow you to monitor and control all crucial cultivation parameters to create a controlled and reproducible process.
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Do I need to buy additional equipment to use the CellScrew®?The CellScrew® is handled just as a regular roller bottle, where it is placed in an incubator on a roller device. However, it is crucial to position the roller bottle at a 10-15 degree angle on the roller to ensure the filter cap remains dry and the system operates effectively. For simultaneous cultivation of three CellScrew® mini in the dimensions of a CellScrew® 6K, the first order of our bundle includes the CellScrew®mini TriDock.
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Is the CellScrew® sterile?Yes, the CellScrew® comes fully sterilized, as it is vacuum sealed and gamma irradiated. We strongly advise against autoclaving the CellScrew® as it can cause damage to the product's structure and integrity.
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