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Our products in detail
Application Notes
October 2022
Process Engineering Characterization of the
CellScrew® 6K & the CellScrew® 10K
Mixing Time and Oxygen Transfer Coefficient
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The CellScrew® offers short mixing times across the entire recommended rpm range
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The CellScrew® exhibits a kLa-value comparable to that of regular multiple-use benchtop bioreactor systems
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As the rotation speed increases, the kLa-value of the CellScrew® rises, and the mixing time decreases
February 2023
Cultivation and expansion of
HEK-293 cells in the CellScrew® 6K
Application of PLA as a growth surface for adherent cells
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Attachment of HEK-293 in the CellScrew® was successful
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Expansion of HEK-293 in the CellScrew® was successful and exceeded DSMZ data
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No protocol modification necessary for trypsinization
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Harvesting enzyme concentration was reduced by 66 % without loss in harvest efficiency
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Can all adherent cell lines be cultivated in the CellScrew®?The CellScrew® has been tested with various cell lines, such as mouse fibroblasts (L-929), human kidney cells (HEK-293), and stem cells (human mesenchymal stromal cells). The adhesion rates, growth rates, and final cell densities observed were comparable to or better than those achieved on standard polystyrene surfaces. For more information on the testing of HEK-293 cells on the PLA surface and the CellScrew®, please refer to this Application Note. Order free material sample 12-well plates to test our surface with your specific cell line.
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Does the CellScrew® have a smaller carbon footprint compared to standard cell culture devices?Yes! The CellScrew® is made from polylactic acid (PLA), which is derived from the polymerization of lactic acid produced from plants. Our additive manufacturing process allows us to produce the same growth surface area with up to 80% less material compared to injection molded culture systems. To comprehensively assess the carbon footprint of the CellScrew®, we are currently conducting a life cycle analysis.
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Is the CellScrew® GMP-certified?The CellScrew® is currently research grade, but we aim to achieve GMP compliance by the end of Q1 2024 and are in the process of constructing a GMP-compliant facility to support this goal.
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Can cells be visualized under the microscope in the CellScrew®?The CellScrew® cannot be microscoped, similar to most other expansion systems that exceed a growth surface of ~300 cm². We recommend using our free 12-well sample plates with inserts to characterize your cell growth and medium on the TC-treated surface.
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Do I need to buy additional equipment to use the CellScrew®?The CellScrew® is handled just as a regular roller bottle, where it is placed in an incubator on a roller device. However, it is crucial to position the roller bottle at a 10-15 degree angle on the roller to ensure the filter cap remains dry and the system operates effectively.
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Is the CellScrew® sterile?Yes, the CellScrew® comes fully sterilized, as it is vacuum sealed and gamma irradiated. We strongly advise against autoclaving the CellScrew® as it can cause damage to the product's structure and integrity.
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