MADE IN GERMANY
The screw in the bottle for the cells in the lab
The CellScrew®'s features unique internal structures, including an Archimedes Screw and concentric cylinders, which create a low-shear stress mixed system. This design facilitates high oxygenation and efficient nutrient exchange, promoting optimal adherent cell attachment and growth.
The CellScrew®'s utilizes an Archimedes Screw that transfers the medium within the bottle by slow rotation. A tube in the middle allows the medium to flow to the bottle's bottom, creating a circular media flow. This, combined with continuous mixing on a roller bottle device, ensures excellent oxygen transfer and optimal cell cultivation conditions for your adherent cell lines.
Multiple concentric cylinders form an extensive growth surface for cells, maximizing the available area and enabling the growth of more adherent cells in one simple system.
The CellScrew® combines the benefits of conventional adherent cell culture systems.
Let's transform the biotech sector with plant-based plastics.
The CellScrew® is made of poly-lactic acid (PLA), a plant-based biopolymer produced entirely from renewable crops.
Consume 80% less plastic!
As the CellScrew® is 3D printed, you can grow cells on a large growth surface area produced from less material, helping to minimize your lab's plastic waste.
Reduce your greenhouse gas emissions by 90%.
Choosing plant-based plastics like PLA contributes to your climate-friendly efforts. The crops used for PLA synthesis remove CO₂ from the atmosphere, and emissions are returned to the earth during the disposal process.
Cell growth & attachment on the PLA surface
Our lab has tested the attachment and growth of HEK-293 cells on the PLA surface in both our sample inserts 12-well plate and the CellScrew®, demonstrating successful cultivation and expansion.
Attachment of HEK-293 in the CellScrew® was successful, and the harvesting enzyme concentration was reduced by 66 % without loss in harvest efficiency, resulting in resource savings.
Can all adherent cell lines be cultivated in the CellScrew®?The CellScrew® has been tested with various cell lines, such as mouse fibroblasts (L-929), human kidney cells (HEK-293), and stem cells (human mesenchymal stromal cells). The adhesion rates, growth rates, and final cell densities observed were comparable to or better than those achieved on standard polystyrene surfaces. For more information on the testing of HEK-293 cells on the PLA surface and the CellScrew®, please refer to this Application Note. Order free material sample 12-well plates to test our surface with your specific cell line.
Does the CellScrew® have a smaller carbon footprint compared to standard cell culture devices?Yes! The CellScrew® is made from polylactic acid (PLA), which is derived from the polymerization of lactic acid produced from plants. Our additive manufacturing process allows us to produce the same growth surface area with up to 80% less material compared to injection molded culture systems. To comprehensively assess the carbon footprint of the CellScrew®, we are currently conducting a life cycle analysis.
Is the CellScrew® GMP-certified?The CellScrew® is currently research grade, but we aim to achieve GMP compliance by the end of Q1 2024 and are in the process of constructing a GMP-compliant facility to support this goal.
Can cells be visualized under the microscope in the CellScrew®?The CellScrew® cannot be microscoped, similar to most other expansion systems that exceed a growth surface of ~300 cm². However, we are currently developing a fully automated, closed production platform for the cellScrew. This platform will allow you to monitor and control all crucial cultivation parameters to create a controlled and reproducible process.
Do I need to buy additional equipment to use the CellScrew®?The CellScrew® is handled just as a regular roller bottle, where it is placed in an incubator on a roller device. However, it is crucial to position the roller bottle at a 10-15 degree angle on the roller to ensure the filter cap remains dry and the system operates effectively.
Is the CellScrew® sterile?Yes, the CellScrew® comes fully sterilized, as it is vacuum sealed and gamma irradiated. We strongly advise against autoclaving the CellScrew® as it can cause damage to the product's structure and integrity.